Aims and objectives
Bone regeneration is an extremely complex process where different factors such as bone morphogenetic proteins,
consistently found in the bone matrix,
play a fundamental role. Many experimentalstudieshavebeenconductedto try toelucidatethe role oftheDemineralizedBoneMatrixintheregenerative processes ofthebone .However,itsuse in realclinicisstillcontroversial andmainlylimitedtothefieldsofTraumatologyand OralSurgery.
Thisis because ofthelackofthoroughandobjectivedataregardingtherealosteogenic...
Methods and materials
The experimental design included 55 male Sprague_Dawley rats to whom a 5 mm (critical size defect) leftparietal craniectomy was performed Fig. 1 . 15 animals were used for training in microsurgical drilling techniques.
The remaining 40 animals were randomly assigned to four different experimental groups according to the biomaterial added to the craniectomy: Control (no biomaterial was added) DBX Colloss Bio-Oss Animals from each experimental group were sacrificed at 12 weeks post surgery....
The 5-mm parietal craniectomy in the left parietal bone of Sprague-Dawley behaved as a critical size defect as defined by Bosch with almost no specimen showing a complete bridging of the bony defect. DBX® and Bio-Oss® were the biomaterials who showed a greater response in terms of bone regeneration enhancement p<0.05.
However the real osteogenic inductor effect was found in the DBX group,
as Bio-Oss apatite particles behave mostly as a osteoconductive framework Fig. 7 Fig. 8 Fig. 9 Cone beam...
The 5-mm parietal craniectomy in rats behaves as a critical size defect,
which is a desirable experimental enviroment to assess the real osteogenic potencial of different biomaterials. DBX®,
a human-derived demineralized bone matrix significantly stimulates bone regeneration in a critical size defect model in rats (p<0,05). The Cavalieri stereology approach to determine the volume of bone regenerated in the craniotomy,
proved to be reliable,
consistent and easy to apply with a reasonably...
López Arcas Calleja 1 , R.
Lorente Ramos 1 , F.
Azpeitia Armán 1 , M.
Grande Barez 1 , J.
Arias Gallo 2 , M.
Burgueño García 2 1.- Radiology Department.Unidad Central de Radiodiagnóstico de la CAM.Hospital Universitario Infanta Leonor (Vallecas). 2.-Maxillofacial SurgeryDepartment.
Hospital Ruber Juan Bravo. . 3.- Maxillofacial SurgeryDepartment.
Hospital Universitario La Paz. MADRID.
Utilización de Colloss® para el tratamiento de quistes mandibulares.Rev Esp Cirug Oral y Maxilofac.
2007; 29:1-11. Nienhuijs ME,
Healing of bone defects in the goat mandible,
using Colloss-E and beta-tricalciumphosphate.
J Biomed Mater Res B Apll Biomater.
2010;92:517-24. Katz JM,
Demineralized bone matrix as an osteoinductive biomaterial and in vitro predictors of its biological...