EANM 20 / EP-266
Quercetin as a potential psychotherapeutic agent in a mouse model with acute exposure to cocaine: a preclinical PET study
Congress: EANM 20
Poster No.: EP-266
Type: Scientific e-Poster
Keywords: Preclinical Studies -> Preclinical Studies -> Preclinical Cardiology and Neurology, 4. Cardiovascular System, 401 Basic Science, 5. Neurosciences, 501 Basic Science,
Authors: F. M. Ribeiro1, C. Nicolucci2, M. Lapo Pais3, A. I. Santos4, P. M. Encarnação1, A. L. Silva1, I. F. Castro5, P. M. Correia1, V. I. Assunção1, J. F. Veloso1, D. Priolli2; 1Institute for Nanostructures, Nanomedelling and Nanofabrication (i3N), University of Aveiro, Aveiro, PORTUGAL, 2Multidisciplinary Research Laboratory, São Francisco University, Bragança Paulista, BRAZIL, 3Faculty of Sciences and Technology, University of Coimbra, Coimbra, PORTUGAL, 4Institute for Clinical and Biomedical Research (iCBR), Center for Innovative Biomedicine and Biotechnology (CIBB), Coimbra, PORTUGAL, 5Radiation Imaging Technologies Lda (RI-TE), Ílhavo, PORTUGAL

Materials & Methods

A total of 16 male Balb/c mice with a mean age of 6-weeks and an average weight of 26.4 g were used in this study. The animals were randomly divided into four groups according to the intraperitoneal (ip) administration of saline (0.9% NaCl), cocaine (0.5 mg/kg), quercetin (50 mg/kg) or cocaine followed by quercetin (referred dosages). 18F-FDG was used to study cerebral metabolic rates of glucose consuption, which were reflected by local radiotracer uptake changes in brain metabolic activity of animals exposed to cocaine, as well as to quercetin. Mice fasted 6-8 hours before PET scan. Awake mice were injected with 18F-FDG (7.5 MBq/0.4mL 0.9% NaCl, ip) and placed in their home cages until the anesthesia. For optimal radiotracer distribution, mice were kept conscious during the uptake period. White noise (80dB) stimuli were performed prior to PET image acquisition being the baseline for controls [6]. After 50 min, they were anesthetized [0.2 mL of a mixture of Ketamine (1.5 mg/mg weight) + Clorpromazine (0.05 mg/mg weight) (3:1) : saline (1:1)] and placed on the bed of the easyPEt.3D scanner (RI-TE Lda, Portugal [7-8]), centered in the FOV. A heating apparatus was connected to the scanner's bed to keep the animals warm. A list-mode PET acquisition of 30 min started 60 min after 18F-FDG injection. The data was reconstructed using a dedicated 3D reconstruction method based on OSEM and MLEM algorithms, considering the original geometry of the scanner and a high number of possible LORs. Digimouse 3D mouse atlas [9-11] was applied for anatomical detail. VOIs were drawn from the same template co-registered with PET data using AMIDE software [12]. SUV was calculated and results were expressed as mean±standard deviation. All experiments were conducted in accordance withe EU directives (86/609/EEC) for the care of laboratory animals and followed the iCBR Vivarium guidelines. The project is under the ORBEA 17/2015 and the DGAV authorization.

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